comparison of biochemical properties of recombinant endoglucanase ii of trichoderma reesei in methylotrophic yeasts, pichia pastoris and hansenula polymorpha

نویسندگان

ali akbarzadeh

seyed omid ranaei siadat

mohammad reza zamani

mostafa motallebi

mohammad barshan tashnizi

چکیده

bioconversion of cellulosic material into bioethanol needs cellulase complex enzymesthat contain endoglucanase, exoglucanase and beta glucosidase. one of the most important organisms that produce cellulases is the filamentous fungi, trichoderma reesei which able to secrete large amounts of different cellulases. these enzymes are probably the most widely used cellulases industrially, however, the cellulases excreted from fungi are not stable at high ph or high temperatures. in this study methylotrophic yeasts, pichia pastoris and hansenula polymorpha were used for the comparative heterologous production of endoglucanase ii. two synthetic egii genes with p. pastoris and h. polymorpha codon preferences were transferred into the yeasts. in addition, both expression vectors contained the pre-pro-sequence of saccharomyces cerevisiae mating factor alpha to allow secretion of protein. enzymes characterization demonstrated increasing thermal stability in both recombinants egii compare with native enzyme from t. reesei and the hansenula enzyme was more stable than pichia in higher temperature. biochemical properties determination on different substrates showed higher binding site affinity in pichia than hansenula and native one. we can conclude that p. pastoris and h. polymorpha are appropriate hosts for expression and production of endoglucanase with improved thermal stability.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Comparison of biochemical properties of recombinant endoglucanase II of Trichoderma reesei in methylotrophic yeasts, Pichia pastoris and Hansenula polymorpha

Bioconversion of cellulosic material into bioethanol needs cellulase complex enzymesthat contain endoglucanase, exoglucanase and beta glucosidase. One of the most important organisms that produce cellulases is the filamentous fungi, Trichoderma reesei which able to secrete large amounts of different cellulases. These enzymes are probably the most widely used cellulases industrially, however, th...

متن کامل

Comparison of biochemical properties of recombinant phytase expression in the favorable methylotrophic platforms of Pichia pastoris and Hansenula polymorpha

Phytic acid is the dominant form of phosphorous in plant seeds. However, phytic acid cannot beutilized by animals and causes them serious phosphate deficiency. Utilization of phytase as ananimal feed additive can affect liberation of phosphor and its mineral availability. In this study,heterologous expression of the A. niger phyA gene was investigated in the methylotrophic yeastsP. pastoris and...

متن کامل

comparison of biochemical properties of recombinant phytase expression in the favorable methylotrophic platforms of pichia pastoris and hansenula polymorpha

phytic acid is the dominant form of phosphorous in plant seeds. however, phytic acid cannot beutilized by animals and causes them serious phosphate deficiency. utilization of phytase as ananimal feed additive can affect liberation of phosphor and its mineral availability. in this study,heterologous expression of the a. niger phya gene was investigated in the methylotrophic yeastsp. pastoris and...

متن کامل

Expression of recombinant staphylokinase in the methylotrophic yeast Hansenula polymorpha

BACKGROUND Currently, the two most commonly used fibrinolytic agents in thrombolytic therapy are recombinant tissue plasminogen activator (rt-PA) and streptokinase (SK). Whereas SK has the advantage of substantially lower costs when compared to other agents, it is less effective than either rt-PA or related variants, has significant allergenic potential, lacks fibrin selectivity and causes tran...

متن کامل

Expression of recombinant proteins in the methylotrophic yeast Pichia pastoris.

Protein expression in the microbial eukaryotic host Pichia pastoris offers the possibility to generate high amounts of recombinant protein in a fast and easy to use expression system. As a single-celled microorganism P. pastoris is easy to manipulate and grows rapidly on inexpensive media at high cell densities. Being a eukaryote, P. pastoris is able to perform many of the post-translational mo...

متن کامل

Cloning and heterologous expression of Laccase in pichia pastoris and determination some of biochemical properties

Laccase (EC 1.10.3.2) are multi-copper oxidase which catalyze the oxidation aromatic and non- aromatic compounds with electron reduction of molecular oxygen to water. Nucleotide sequence of laccase (accession number : ) was optimized according codon preference of Pichia pastoris. Gene was synthesized and cloned into pPICZalpha A. laccase under control of AOX1 promoter was transformed to P.pasto...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
progress in biological sciences

ناشر: university of tehran

ISSN 1016-1058

دوره 3

شماره 1 2013

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023